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| R odriguez,Andrea P.; Tsujigiwa,Hidetsugu; Gunduz,Mehmet; Cengiz,Beyhan; Nagai,Noriyuki; Tamamura,Ryo; Borkosky,Silvia S.; Takagi,Tohru; Inoue,Miho; Nagatsuka,Hitoshi. |
| Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MDPC-23 cells; KUSA/A1 cells; Diffusion chamber; RT-PCR analysis; Immunohistochemical analysis. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100005 |
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| Borkosky,Silvia S.; Nagatsuka,Hitoshi; Orita,Yorihisa; Tsujigiwa,Hidetsugu; Yoshinobu,Junko; Gunduz,Mehmet; Rodriguez,Andrea P.; Missana,Liliana R.; Nishizaki,Kazunori; Nagai,Noriyuki. |
| The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during postnatal stage (PN) 1, PN3, PN5, PN10 and PN14 by using in situ hybridization. Positive Notch1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Notch signaling; Odontogenesis; Cell differentiation; Molar morphogenesis. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300006 |
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